Videos

Gunther Gerisch's Demonstration Movies
Chemotaxis of neutrophil chasing a bacterium
Chemotaxis of neutrophil chasing bacterium.
Micropipet-mediated aggregation
Micropipet-mediated aggregation
Single Dictyostelium cell exposed to a cyclic AMP gradient from micropipette
Single Dictyostelium cell exposed to a cyclic AMP gradient from a micropipette. Concentration of cyclic AMP changes ~20% across the cell.
Stepwise chemotactic movements of Dictyostelium cells towards an aggregation center
Stepwise chemotactic movements of Dictyostelium cells towards an aggregation center.
GFP-Tagged Chemoattractant Receptors and G-protein Subunits in Chemotaxing D. discoideum Cells
Colorized confocal image of GFP-Gb in chemotaxing cells.
Colorized confocal image of GFP-Gb in chemotaxing cells. Gb-GFP was used to rescue the null phenotype of Gb null cells.
Green fluorescent protein-tagged receptors
Green fluorescent protein-tagged receptors in living cells undergoing chemotaxis.
PH-GFP Biosensors Reporting PIP3 Levels in Chemotaxing D. discoideum Cells
Cells expressing PH-GFP carrying out phagocytosis of yeast particles
Cells expressing PH-GFP carrying out phagocytosis of yeast particles. Total time of video is ~4 minutes.
Same cells as 9 treated with LY429033
CRAC-GFP translocates to the leading edge of newly elicited pseudopods in chemotactically moving cells. Cells treated with LY429033. The micropipette is located in the center of the converging cells.
Dynamics of the CRAC-GFP redistribution
Dynamics of the CRAC-GFP redistribution. A large increment in chemoattractant was applied by increasing the microinjector pressure and the cells immediately respond. As the gradient of cAMP is restored, the polarization of CRAC-GFP is re-established.
Kinetic analysis of translocation of CRAC-GFP
Kinetic analysis of translocation of CRAC-GFP in response to a uniform increase in chemoattractant concentration. Frames are taken every two seconds and chemoattractant was added just before cell goes out of focus.
Latrunculin A immobilized cells
Latrunculin A immobolized cells were exposed to a pipette in upper portion of field. The pipette was then moved around the cells and the binding sites for PH-domains move around the cell on the inner face of the membrane.
Latrunculin immobilized cell was exposed to two opposing cyclic AMP gradients
Latrunculin immobilized cell was exposed to two opposing cyclic AMP gradients (see two micropipettes). The strength of the two gradients was alternated during the video.
Latrunculin immobilized cells were stimulated
Latrunculin immobilized cells were stimulated with a uniform increment in cyclic AMP.
Latruncin immobilized cells exposed to micropipette containing cyclic AMP
Latrunculin immobolized cells were exposed to a micropipette containing cyclic AMP.
Polarized translocation of PH-GFP in highly polarized cells
Polarized translocation of PH-GFP in highly polarized cells. Micropipette was located in the lower region beyond the field. Cells were rapidly exposed to a saturating dose of attractant.
Response of CRAC-GFP to an approaching wave of chemoattractant-left side
Response of CRAC-GFP to an approaching wave of chemoattractant. Pipette located on the left side of the same cell as in #6 was briefly turned on for six seconds at three times during the sequence.
Response of CRAC-GFP to an approaching wave of chemoattractant
Response of CRAC-GFP to an approaching wave of chemoattractant. Pipette located on the right side of the cell was briefly "turned on" for six seconds at three times during the sequence.
Translocation of CRAC-GFP in cells lacking actin filament formation
Translocation of CRAC-GFP in cells lacking actin filament formation. Cells chemotaxing towards pipette in lower portion of field were poisoned with Latrunculin A and followed for the next several minutes.
Single Molecule Imaging by Masahiro Ueda and Toshio Yanagida
Pten cells treated with 30um mg of LY429033
Chemotaxis of PTEN cells towards cyclic AMP filled micropipette in upper right hand corner of field. Cells are expressing PH-GFP.

Cells are treated with 30 μm mg of LY429033.

Single molecule imaging of PH-GFP molecules
Single molecule imaging of PH-GFP molecules moving from the cytosol to the plasma membrane on the basal surface of the cell.
Single molecule imaging of the binding of CY3-cyclic AMP to the basal surface of a cell
Single molecule imaging of the binding of CY3-cyclic AMP to the basal surface of a cell. Lifetime of binding events is ~1.5 seconds.
Role of PTEN in Directional Sensing
Chemotaxis of pten cells
Chemotaxis of PTEN cells towards cyclic AMP filled micropipette in upper right hand corner of field. Cells are expressing PH-GFP.
Chemotaxis of wild-type cells
Chemotaxis of wild-type cells expressing PH-GFP towards cyclic AMP filled micropipette in lower left hand corner of field.
Distribution of PTEN-GFP in cells chomtaxing towards cyclic AMP
Distribution of PTEN-GFP in cells chemotaxing towards cyclic AMP. Note that PTEN localizes to the back of the cell.
Pten cells expressing PH-GFP exposed to a cyclic AMP gradient coming from a micropipette in the middle of the field
Pten cells expressing PH-GFP exposed to a cyclic AMP gradient coming from a micropipette in the middle of a field.
Pten cells expressing PH-GFP
PTEN cells expressing PH-GFP. Cells were exposed to a sudden increase in cyclic AMP (white flash). Total time of video represents ~3.5 minutes of stimulation.