Genetic and Systemic Dissection of Signaling Network


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Which genetic components are involved in specific signal transduction cascades? How do cells coordinate numerous signaling and cytoskeletal events via internal feedback loops? How do cells decide when and where to activate a specific signaling event so that they can polarize and migrate correctly? How do cells resolve different conflicting environmental cues and determine how to spatiotemporally regulate stochastic activities? These are some questions we are focused on.


Relevant publications and preprints:

  • Kuhn J, Banerjee P, Haye A, Robinson DN, Iglesias PA, and Devreotes PN. 2024. Complementary Cytoskeletal Feedback Loops Control Signal Transduction Excitability and Cell Polarity. bioRxiv [Preprint]. DOI: 10.1101/2024.02.13.580131.

  • Lin Y*, Pal DS*, Banerjee P, Banerjee T, Qin G, Deng Y, Borleis J, Iglesias PA and Devreotes PN. 2024. Ras suppression potentiates rear actomyosin contractility-driven cell polarization and migration. Nat Cell Biol 26(7):1062-1076. DOI: 10.1038/s41556-024-01453-4.

  • Pal DS, Banerjee T, Lin Y, de Trogoff F, Borleis J, Iglesias PA, Devreotes PN. 2023. Actuation of single downstream nodes in growth factor network steers immune cell migration. Dev Cell 58(13):1170-1188.e7. DOI: 10.1016/j.devcel.2023.04.019.

  • Banerjee T, Biswas D*, Pal DS*, Miao Y, Iglesias PA, and Devreotes PN. 2022. Spatiotemporal dynamics of membrane surface charge regulates cell polarity and migration. Nat Cell Biol 24, 1499–1515 (2022). DOI: 10.1101/2022.05.19.492577.

  • Miao Y, Battacharya S, Banerjee T, Abudaker-Sharif B, Long Y, Inoue T, Iglesias P, and Devreotes PN. 2019. Wave patterns organize cellular protrusions and control cortical dynamics. Mol Syst Biol 15(3):e8585. DOI: 10.15252/msb.20188585.

  • Edwards M, Cai H, Abubaker-Sharif B, Long Y, Lampert T, and Devreotes PN. 2018. Insight from the maximal activation of the signal transduction excitable network in Dictyostelium discoideum. Proc Natl Acad Sci U S A 115(16):E3722-E3730. DOI: 10.1073/pnas.1710480115.

  • Lampert TJ, Kamprad N, Edwards M, Borleis J, Watson AJ, Tarantola M, Devreotes PN. 2017. Shear force-based genetic screen reveals negative regulators of cell adhesion and protrusive activity. Proc Natl Acad Sci U S A 114(37) E7727-E7736. DOI: 10.1073/pnas.1616600114.

  • Miao Y, Bhattacharya S, Edwards M, Cai H, Inoue T, Iglesias P, Devreotes PN. 2017. Altering the threshold of an excitable signal transduction network changes cell migratory mode. Nat Cell Biol 19(4):329-340. DOI: 10.1038/ncb3495.

  • Cai H, Katoh-Kurasawa M, Muramoto T, Santhanam B, Long Y, Li L, Ueda M, Iglesias PA, Shaulsky G, and Devreotes PN. 2014. Nucleocytoplasmic shuttling of a GATA transcription factor functions as a development timer. Science 343(6177):1249531. DOI: 10.1126/science.1249531.

  • Huang C-H,Tang M, Shi C, Iglesias PA, and Devreotes PN. 2013. An excitable signal integrator couples to an idling cytoskeletal oscillator to drive cell migration. Nat Cell Biol 15(11):1307-16. DOI: 10.1038/ncb2859.

  • Xiong Y, Huang C-H, Iglesias PA, and Devreotes PN. 2010. Cells navigate with a local-excitation, global-inhibition-biased excitable network. Proc Natl Acad Sci U S A, 107:17079-17086. DOI: 10.1073/pnas.1011271107.