RBD-GFP and LimE-RFP in undulations and protrusions

Time-lapse TIRF video of a Dictyostelium cell co-expressing RBD-GFP and LimE-RFP with a rate of 2 spf. Cytoplasmic fluorescence of RBD-GFP and LimE-RFP was subtracted to highlight areas of increased intensity. Cell boundary (in gray) was derived from the cytoplasmic fluorescence of RBD-GFP. The video is shown at 7 frames/s.