Cells expressed myr-FKBP-FKBP and mCherry-FRB-Inp54p. Rapamycin was added at time 6 min. Examples of amoeboid-to-fan-shaped and amoeboid-to-oscillatory transitions were outlined, with blue, green, and red representing amoeboid, fan-shaped, and oscillatory, respectively. Scale bar represents 20 μm. Images were acquired every 12 sec and the video is shown at 20 frame/sec.